Hormone Binding in Plants

INTRODUCTION ,,', ,', 268 THE HORMONE· RECEPTOR CONCEPT ', 268 Methods of Assessing Hormone-Receptor Interactions 268 Kinetic Analysis of Hormone Binding ....... ........ ......... ,,. 269 Criteria for Physiologically Meaningful Binding 270 Structural specificity '''''' ..... ,''''', ..... ,''', ,'''''', ........ 270 Kinetic parameters ..... ........ ,,,,.,, ....... ,,,',, 270 Tissue specificity ....... ....... ,''', ........ ..... 270 Binding of Hormones to Cellular Components Other Than Receptors ...... 271 AUXINS ,', ,', ,', ,' ,''''', , ,', ,', ,,.. 271 Autoradiographic Studies ....... ......... ,''','', ..... ........ ,,, 272 Auxin and DNA '. ..... ,'. ..... ,',, ''''''' 272 Interaction of Auxin with RNA ....... ........ 272 Auxin and Nuclei ''' ''''', ''..''''. ,, ......... ,,'',, 274 Auxin and RNA Synthesis ....... ,, 274 Auxin and Protein ........ ........ ... ..., 276 Auxin-Lipid Interactions in Model Systems ,,,,. 277 Binding to Particulate Sub-Cellular Fractions ..... 278 Naphthylphthalamic acid ........ ......... 278 Auxin .... .,, ........ .. ,.. 279 GIBBERELLINS 281 Synthesis of Radioactive Gibberellins ......... ..... 281 In Vivo Binding Studies ,,,,.,,...... 282 In Vitro Binding Studies 282 Model Systems : 283 CYTOKININS 283 Synthesis of Radioactive Cytokinins ....... ....... ..... ......... 283 In Vivo Binding of Cytokinins ..... .,,,,...... 284 In Vitro Binding of Cytokinins . ,,,, 284