Fermentation of single and mixed substrates by the parent and an acid‐tolerant, mutant strain of Clostridium Thermoaceticum

Growth of the parent and acid-tolerant mutant strains of Clostridium thermoaceticum was examined on a variety of substrates and mixtures of substrates. Nondiauxic growth was noted for both strains on combinations of carbohydrates, organic acids, or a carbohydrate and an organic acid. The mutant strain was able to grow on DL-lactate as sole energy source. The parent strain would not grow on lactate as sole energy source but consumed lactate when presented with a second fermentable substrate. Neither strain would grow on formate as sole energy source, but both consumed formate when presented with a second fermentable substrate.