Key result
Rotavirus was detected in 12.3% of children aged 0-59 months with acute gastroenteritis, revealing a high diversity of genotypes including G1P[8], G12P[8], and G3P[6] (9.8% each).
Why the study?
This study aimed to determine the circulating rotavirus genotypes among children 0 to 59 months with acute gastroenteritis in Zaria, Northwestern Nigeria.
Cross-Sectional (n=415)
Yes
The study identified a high diversity of rotavirus strains, including unusual genotypes and mixed infections, in children with acute gastroenteritis in Northwestern Nigeria, which may impact vaccine effectiveness.
Answers are grounded in this paper and cite their sources.
May warrant enhanced rotavirus surveillance in Nigeria; leaves open whether genotype diversity compromises vaccine effectiveness.
Objective This study aimed to determine the circulating rotavirus genotypes among children 0 to 59 months with acute gastroenteritis in Zaria, Northwestern Nigeria. Methods A cross-sectional hospital-based study was performed from November 2015 to April 2017 on 415 stool samples of children 0 to 59 months with acute gastroenteritis presenting at the pediatric clinics in five selected health care facilities in Zaria, Northwestern Nigeria. The stool samples were tested for group A rotavirus antigen by enzyme-linked immunosorbent assay (ELISA) using the ProSpecT™ Rotavirus Microplate Assay (Thermo Scientific Oxoid, United Kingdom). Rotavirus-positive strains were further characterized using seminested multiplex reverse transcriptase polymerase chain reaction. Results Of the 415 diarrheal stool samples collected from children with acute gastroenteritis, 12.3% (n = 51) tested positive by ELISA. Five rotavirus G genotypes (G1, G2, G3, G9, and G12) and three P genotypes (P[4], P[6], and P[8]) were detected. Eight genotype combinations were detected with G1P[8], G12P[8], and G3P[6] being the most prevalent (9.8% each) followed by G1P[6], G2P[4], and G9P[8] (5.9% each). G/P mix types representing 25.5% (n = 13) of strains were detected during the study period. Conclusion This study found a high diversity of rotavirus strains with emerging unusual rotavirus genotypes and a concurrent high proportion of mixed infections. This increased antigenic diversity of rotavirus strains may impact on vaccine effectiveness and rotavirus epidemiology and highlights the importance of continuous surveillance.
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Giwa et al. (2021) conducted a cross-sectional in acute gastroenteritis (n=415). Rotavirus infection was evaluated on Rotavirus positivity and genotype distribution. Rotavirus was detected in 12.3% of children aged 0-59 months with acute gastroenteritis, revealing a high diversity of genotypes including G1P[8], G12P[8], and G3P[6] (9.8% each).
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