Key result
Restriction enzyme analysis of unintegrated MAV viral DNAs and their integrated proviruses suggests they have a direct terminal redundancy of ~0.3 megadaltons and integrate colinearly.
The study characterizes the DNA intermediates of avian myeloblastosis-associated viruses, revealing specific restriction enzyme patterns and integration characteristics.
MAV integration patterns mapped in avian models; leaves open whether mechanisms extend to human retroviruses.
The major species of unintegrated linear viral DNA identified in chicken embryonic fibroblasts infected with either the avian myeloblastosis-associated viruses (MAV-1, MAV-2) or the standard avian myeloblastosis virus complex (AMV-S) has a mass of 5.3 X 10(6) daltons. An additional minor DNA component observed only in AMV-S-infected cells has a mass of 4.9 X 10(6) daltons. The unintegrated linear viral DNAs and integrated proviruses of MAV-1 and MAV-2 have been analyzed by digestion with the restriction endonucleases EcoRI and HindIII. MAV-2 lacks a HindIII site present in MAV-1. These fragments have been compared to those generated by EcoRI and HindIII digestion of linear viral DNAs of AMV-S. Restriction enzyme digestion of AMV-S viral DNA produced unique fragments not found with either MAV-1 or MAV-2 viral DNAs. The major viral component present in AMV-S stocks has the HindIII restriction pattern of MAV-1. Restriction enzyme analysis of the 5.3 X 10(6)-dalton unintegrated MAV viral DNAs and their integrated proviruses suggests that the DNAs have a direct terminal redundancy of approximately 0.3 megadaltons and integrate colinearly with respect to the unintegrated linear DNA.
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Bergmann et al. (1980) studied this question. Restriction enzyme analysis of unintegrated MAV viral DNAs and their integrated proviruses suggests they have a direct terminal redundancy of ~0.3 megadaltons and integrate colinearly.
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