Key result
Adipocytes from high-fat diet-fed rats showed lower insulin-induced glucose uptake and adipokine secretion compared to controls, while vitamin C modified responses independently of dietary origin.
Why the study?
Does vitamin C and glucose concentration alter the response to insulin in adipocytes from rats fed a high-fat diet?
Does vitamin C and glucose concentration alter the response to insulin in adipocytes from rats fed a high-fat diet?
In vivo high-fat diet intake determines a differential response to insulin and glucose in rat adipocytes, while vitamin C modifies responses independently of previous dietary intake.
Answers are grounded in this paper and cite their sources.
Animal adipocyte findings link high-fat diet to impaired insulin responses modifiable by vitamin C; leaves open human translation.
Antioxidant-based treatments have emerged as novel and interesting approaches to counteract fat accumulation in obesity and associated metabolic disturbances. Adipocytes from rats that were fed on chow or high-fat diet (HFD) for 50 d were isolated (primary adipocytes) and incubated (72 h) on low (LG; 5.6 mmol/L) or high (HG; 25 mmol/L) glucose levels, in the presence or absence of 1.6 nmol/L insulin and 200 μmol/L vitamin C (VC). Adipocytes from HFD-fed animals presented lower insulin-induced glucose uptake, lower lactate and glycerol release, and lower insulin-induced secretion of some adipokines as compared with controls. HG treatment restored the blunted response to insulin regarding apelin secretion in adipocytes from HFD-fed rats. VC treatment inhibited the levels of nearly all variables, irrespective of the adipocytes' dietary origin. The HG treatment reduced adipocyte viability, and VC protected from this toxic effect, although more drastically in control adipocytes. Summing up, in vivo chow or HFD intake determines a differential response to insulin and glucose treatments that appears to be dependent on the insulin-resistance status of the adipocytes, while VC modifies some responses from adipocytes independently of the previous dietary intake of the animals.
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García‐Díaz et al. (2012) studied Obesity and associated metabolic disturbances. High-fat diet (in vivo) and in vitro glucose, insulin, and vitamin C vs. Chow diet (in vivo) and control in vitro conditions was evaluated on Glucose uptake, lactate and glycerol release, adipokine secretion, and adipocyte viability. Adipocytes from high-fat diet-fed rats showed lower insulin-induced glucose uptake and adipokine secretion compared to controls, while vitamin C modified responses independently of dietary origin.
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