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SUMMARYCellulolytic enzymes are produced by Poronia oedipus grown on soluble and insoluble forms of cellulose. Cellulase production is negligible when the organism is grown in the presence of glucose. Optimal cellulolytic activity occurs at 50–55 C. Hydrolysis of filter paper is greatest at pH 4.2; but with carboxymethylcellulose as a substrate, optimal cellulase activity occurs at pH 4.4 to 5.0. Total accumulation of reducing sugar in the cellulase assay with filter paper as a substrate does not rise above 1% of the substrate concentration; with CMC as a substrate, approximately 5% conversion is reported.
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Denison et al. (1977) studied this question.
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