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The effect of Cercospora beticola toxin (CBT) on ATP‐dependent and nigericin‐induced proton translocation, monitored by acridine orange uptake in pea stem microsomal vesicles, was studied. CBT inhibits ATP‐dependent proton translocation, but not the nigericin‐induced H+/K+ exchange. The inhibitory effect is dependent on CBT concentration, time of preincubation with CBT and protein concentration of the vesicle suspension. The previously observed effects of CBT on membrane transport phenomena, in the light of the present results, are in agreement with the hypothesis that the primary effect of the toxin is exerted on an ATPase of plasmalemma and/or tonoplast, acting as a proton pump.
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Macrı̀ et al. (1983) studied this question.
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