Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance

The current data is dedicated to the study of bioballistic and Agrobacterium -mediated transformation of finger millet with the constructs carrying the mutant α-tubulin gene ( TUAml ), isolated from R-biotype goosegrass ( Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 μM of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgenic nature of plants. The analysis of seed of T 1 of transgenic lines confirmed heterozygous character of inheritance of trifluralin resistance.