The liquid chromatographic separation of catecholamine acidic metabolites using tetrabutylammonium bromide as ion interaction agent is investigated. The effect of several experimental variables, such as mobile phase pH, organic modifier concentration, ion interaction agent concentration, and temperature is analyzed. The mobile phase composition that produces the best chromatographic resolution for the analysis of the compounds of interest is determined. The hydrodynamic voltammogram of the analytes showed that good selectivity can be achieved with electrochemical detection, allowing for the analysis of different compounds at different applied potentials. The Signal-to-Noise ratio is studied as a function of the applied potential.
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Ribotta et al. (1998) studied this question.
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