Key result
Re-evaluation of 800 preselected PA-positive sera revealed that one-third were PA-negative upon retesting, indicating PA should be combined with other methods for anti-HTLV-1 antibody detection.
Why the study?
What is the diagnostic performance and reliability of the particle agglutination assay for anti-HTLV-1 antibody compared to other methods?
Observational (n=800)
Yes
What is the diagnostic performance and reliability of the particle agglutination assay for anti-HTLV-1 antibody compared to other methods?
The particle agglutination assay for anti-HTLV-1 antibody has significant false-positive rates and should be used in combination with other methods for confirmation.
Answers are grounded in this paper and cite their sources.
PA may overestimate HTLV-1 seroprevalence via false positives; leaves open the optimal confirmatory assay combination.
In order to test the performance of particle agglutination assay (PA), 800 preselected PA-positive sera at 8 blood centers in the Kyushu area were tested in various assays. Most blood centers should improve their PA technique, since a third of the samples were PA-negative in our hands. A third of our PA high-titer sera were negative in indirect immunofluorescence and enzyme immunoassay, the results of which were consistent with each other. Western blots did not detect every positive serum. PA inhibition positivity was not consistent with PA titer. Most IgM antibody-positive sera also contained IgG antibody. PA should be used in combination with other methods before notifying the results to positive testees.
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Maeda et al. (1989) conducted an observational in Anti-HTLV-1 Antibody Positivity (n=800). Particle agglutination assay (PA) vs. Indirect immunofluorescence, enzyme immunoassay, and Western blot was evaluated on Assay concordance and performance. Re-evaluation of 800 preselected PA-positive sera revealed that one-third were PA-negative upon retesting, indicating PA should be combined with other methods for anti-HTLV-1 antibody detection.
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