Morphogenetic Studies with Partially Synchronized Cultures of Carrot Embryos

Callus tissue, derived from petiole segments of the wild carrot and containing undifferentiated meristems, can be suspended in liquid nutrient media and passed through a series of sieves to separate the meristems by size. The small meristems of fairly uniform size thus obtained will grow on defined media where their controlled differentiation into embryos can be studied.