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February 13, 2025Discover NanoOpen Access

Eco-friendly synthesis of ZnO, CuO, and ZnO/CuO nanoparticles using extract of spent Pleurotus ostreatus substrate, and their antioxidant and anticancer activities

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Authors

SNSimangele C. NgwenyaNSNkanyiso J. SitholeKRKhosi Ramachela

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Overview

In vitro study reveals enhanced antioxidant activity and cytotoxic effects against HeLa cancer cells from mushroom-derived metal oxide nanoparticles, suggesting potential biomedical utility.

Key Points

  • To synthesize zinc oxide, copper oxide, and composite nanoparticles using spent Pleurotus ostreatus mushroom substrate and assess their antioxidant and anticancer properties.
  • Synthesized ZnO, CuO, and ZnO/CuO nanoparticles using aqueous extracts from spent Pleurotus ostreatus substrate, characterizing them via XRD and UV-vis spectroscopy.
  • Evaluated free radical scavenging using a DPPH antioxidant assay and measured cytotoxicity across human embryonic kidney (HEK 293) and cervical carcinoma (HeLa) cell lines.
  • All nanoparticles demonstrated stronger DPPH radical scavenging than ascorbic acid (IC50 of 4.25 µg/mL), with IC50 values of 2.15 µg/mL for ZnO, 2.16 µg/mL for CuO, and 3.18 µg/mL for ZnO/CuO NPs.
  • Nanoparticles induced dose-dependent cytotoxicity in both tested cell lines, with ZnO NPs demonstrating the highest potency with an IC50 of 1.94 μM in HEK 293 cells and 3.23 μM in HeLa cells.

Cite This Study

Ngwenya et al. (2025) studied this question.

synapsesocial.com/papers/6a9a2ecbcbeb9500cf45afe1https://doi.org/10.1186/s11671-025-04199-6
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