Prevalence of measles in vaccinated and non-vaccinated children

Collection of samples and serum separation From Faisalabad, a total of 1053 children under 10 years of age were surveyed from September, 2013 to May, 2014 about vaccination and disease history of measles. Out of these, 182 blood samples (132 from vaccinated and 50 from non-vaccinated individuals) were collected randomly. Similarly during the same period, from Jhang, 813 children under 10 years of age were surveyed during the same period about vaccination and disease history of measles. Out of these, 182 blood samples (132 from vaccinated and 50 from non-vaccinated individuals) were collected randomly. The blood was collected from each individual in 2.5 or 5ml disposable syringes. Each blood sample was transferred immediately to separate serum clot activator tube (Lab Vac Gel and Clot Activators), kept on ice and shifted to Department of Microbiology, Govt. College University, Faisalabad. Serum was separated from each blood sample by centrifugation of serum collection tubes at 1000-2000 Xg for 3-5 minutes and stored in 1.5ml Eppendorf tubes at -20 C.