When Azotobacter chroococcum grows on glycolic acid as sole C source, it cannot utilize N2 and must be provided with reduced nitrogen. Glycolic acid is metabolized via Kornberg's dicarboxylic acid cycle. The TCA cycle enzymes are low in activity, and isocitric dehydrogenase is absent. It is likely that isocitric dehydrogenase is the source of reductant for nitrogen fixation by Azotobacter nitrogenase.
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Kurz et al. (1973) studied this question.
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