Relationship of 3′,5′-Adenosine Monophosphate Accumulation to Parathyroid Hormone Release in Dispersed Cells from Pathological Human Parathyroid Tissue*

We studied the effects of calcium on cellular cAMP and parathyroid hormone (PTH) release in dispersed parathyroid cells from patients with primary hyperparathyroidism. In four cell preparations there was a statistically significant relationship between calcium-induced changes in these two variables. In three others there was no such correlation. In two of these cAMP content was decreased more than PTH release at high calcium concentrations (2–3 mM) (45% and 85% us. 0%and 30% respectively). In a third PTH release was inhibited 30% or more without any change in cAMP levels. The relationships between basal and agonist-stimulated cAMP accumulation and hormone release were also complex. In three cases, there was a good agreement between the concentration of calcium-inhibiting isoproterenol-stimulated cAMP content and PTH release. On the other hand, in one of these cases and in an additional one there was no clear relationship between the effects of calcium on basal and agonist-stimulated cAMP accumulation. The...