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Free and total Mn(II) content of hepatocytes from fed and fasted rats has been determined by EPR. The difference spectra of cumulative scans of cell samples and the mother medium allows a sensitivity of 0.1 PM free Mn(II). The free and total Mn(II) content was 0.71 2 0.10 and 34.4 f 4.4 nmol/ml of cell H20 in hepatocytes from fed animals and was 0.25 -+, 0.15 and 35.9 +. 7.6 nmol/ml of cell HzO, respectively, in hepatocytes from fasted animals. These results demonstrate a 2.8-fold decrease in free Mn(I1) with no change in the total cell Mn(II) in response to fasting.
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Ash et al. (1982) studied this question.
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