Prostanoid Production in Rabbit Corpus Cavernosum. II. Inhibition of Oxidative Stress

No AccessJournal of UrologyInvestigative Urology1 Sep 1996Prostanoid Production in Rabbit Corpus Cavernosum. II. Inhibition of Oxidative Stress Jennifer T. Daley, Michael T. Watkins, Michael L. Brown, Victoria Martinez, Pedro Cuevas, and Inigo Saenz de Tejada Jennifer T. DaleyJennifer T. Daley , Michael T. WatkinsMichael T. Watkins , Michael L. BrownMichael L. Brown , Victoria MartinezVictoria Martinez , Pedro CuevasPedro Cuevas , and Inigo Saenz de TejadaInigo Saenz de Tejada View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65744-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To investigate the effects of reoxygenation following hypoxia on prostanoid production in rabbit penile corpus cavernosum tissue (RCC) in organ culture. Materials and Methods: Strips of RCC were incubated in organ culture media under either 21 percent O2 (control, PO2 approximately 167 mm.Hg) or 0 percent O2 (hypoxia, PO2 approximately 27 mm.Hg) followed by a reoxygenationi period with 21 percent O2, in the presence or absence of exogenous arachidonate, Tiron or catalase. Prostanoids were measured in collected media by radioimmunoassay. Malondialdehyde levels were measured in RCC following exposure to either control or hypoxia-reoxygenation conditions. Results: Under hypoxic conditions, basal release of prostanoids (PGI2, PGF2 alpha, PGE2 and TXB2) was inhibited. Although this inhibition was reversible upon reoxygenation, the recovery was delayed, requiring at least 2 hours of exposure to 21 percent oxygen to reestablish prostanoid production. Reoxygenation also caused lipid peroxidation as measured by an increase in malondialdehyde levels. When reoxygenation was done in the presence of exogenous arachidonate, recovery of PGI2 production was complete by 1 hour. Reoxygenation in the presence of a scavenger of reactive oxygen species (Tiron) or catalase significantly improved the recovery rate of PGI2 production. Conclusions: These results show that reoxygenation of hypoxic tissue generates oxidative stress that interferes with the recovery of prostanoid production by alteration of a biosynthetic point(s) upstream from prostaglandin H synthase (PGHS) including, at least, phospholipid peroxidation. References 1 : Free radical and ischemic tissue injury. Trends Pharmacol. Sci.1990; 11: 161. Google Scholar 2 : The biphasic effect of oxygen radicals on prostaglandin production by rat mesangial cells. Am. J. Physiol.1987; 252: F743. Google Scholar 3 : Effect of hydrogen peroxide on prostaglandin production and cellular integrity in cultured porcine endothelial cell. J. Clin. Invest.1985; 76: 295. 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Volume 156Issue 3September 1996Page: 1169-1173 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Jennifer T. Daley More articles by this author Michael T. Watkins More articles by this author Michael L. Brown More articles by this author Victoria Martinez More articles by this author Pedro Cuevas More articles by this author Inigo Saenz de Tejada More articles by this author Expand All Advertisement PDF downloadLoading ...