A new type of polymer support for immobilizing urease was investigated. The starting copolymers which grafted acrylic acid onto polyethylene (PE‐g‐AA) were prepared via gamma ray irradiation induced grafting under controlled conditions by postirradiation procedures. The optimum conditions for coupling urease to PE‐g‐AA were established and the physical properties of the support affecting the immobilized enzyme activity were studied. A water soluble carbodiimide (CMC) was used as a condensing agent to immobilize urease. The activities of the resulting enzyme membranes were very high. The hydrophilicity of the support and the activity of the enzyme membrane were proportional to the degree of grafting. Its linear detecting range was 60–400 ppm.
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Hsiue et al. (1990) studied this question.
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