A Transplantation Assay for Mouse Cells Responsive to Antigenic Stimulation by Sheep Erythrocytes.

When cell suspensions containing normal mouse spleen or lymph node cells mixed with sheep erythrocytes are injected into heavily-irradiated mice, foci of hemolysin-producing cells are formed in the spleens of the recipients. A method is described for demonstration of these foci, and evidence is presented that they result from the proliferation of antigen-sensitive precursors of hemolysin-producing cells. The demonstration of the existence of “antigen-sensitive” cells provides strong support for the view that cell proliferation is involved in the generation of hemolysin-producing cells.