Stability and Photochemistry of Vitamin D 2 in Model System

Oxidation of vitamin D 2 in a model system of 12% water and 88% acetone in the presence of 15 ppm riboflavin under light and dark was studied by measuring the depleted headspace oxygen. Riboflavin accelerated the oxidation of vitamin D 2 by singlet oxygen under light, but did not affect the vitamin D 2 oxidation under dark. The effect of 0 and 15 ppm riboflavin on the stability of vitamin D 2 during storage at 25 and 60°C was studied by measuring the contents of vitamin D 2 during 48h. Results indicated that photosensitized singlet oxygen oxidation of vitamin D 2 under light was temperature‐independent, and triplet oxygen oxidation of vitamin D 2 both under light and in the dark was temperature‐dependent.