Comparison between two procedures for stereospecific analysis of triacylglycerols from vegetable oils—I: Olive oil

Two methods for stereospecific analysis of triacylglycerols are compared. Procedure A, based on stereospecific phosphorylation ofsn‐1,2‐diacylglycerols to phosphatidic acids, and procedure B, based on separation of the diastereomeric 1,2(2,3)‐diacylglycerol urethane derivatives by high‐performance liquid chromatography on silica, were applied to olive oil triacyl‐sn‐glycerols. Statistical evaluation of the results showed good reproducibility, and Student'st‐test indicates no statistical differences between the two considered procedures, although some small differences were observed and discussed. Fifteen samples of extra‐virgin olive oil, produced in the same region (Umbria, Italy), were analyzed with the two considered procedures.