A novel multiplex serological immunoassay for health monitoring in rodents

Health monitoring of laboratory rodents is essential for ensuring animal welfare, meeting regulatory requirements for zoonosis control, and maintaining the integrity and reproducibility of scientific studies. Routine serological screening, conducted in accordance with FELASA guidelines, plays a critical role in the detection of infectious agents. While conventional serological assays typically target individual pathogens, existing multiplex platforms often require specialized equipment and technically demanding protocols, limiting their routine use within animal facilities.In this study, we evaluated the performance and usability of a multiplex ELISA-like assay (MIRIAD®, GD Biotech, France), designed for in-house serological surveillance of laboratory rodents. A total of 68 mouse and 27 rat serum samples were analysed, and the results were compared with two commercially available single-target ELISAs, one for mice (Charles River, Wilmington, MA, USA) and one for rats (Biotech Trading Partners, Encinitas, CA, USA). Concordance rates ranging from 94% to 100% were observed across all 26 target pathogens tested, reflecting high sensitivity and sensitivity. Compatibility with dried blood spot (DBS) cards was also evaluated, demonstrating reliable antibody detection after storage and elution, and supporting the potential for reduced sample volumes and simplified sample handling.These findings highlight the multiplex ELISA-like assay as a practical and efficient tool for routine serological health monitoring in laboratory animal facilities. Its ease of use, minimal sample requirements, and compatibility with DBS technology can facilitate more frequent testing, support early detection of pathogens, and promote adherence to the 3Rs (Replacement, Reduction, and Refinement) principles through refinement of health-monitoring practices.