Role of Cutinase in the Penetration of Apple Leaves byVenturia inaequalis

Cutin hydrolysate induced the production of extracellular cutinase by mycelium and germinating conidia of Venturia inaequalis. The level of production was dose-dependent up to 0.25 mg ml -1 of hydrolysate added. Glucose was found to act as a repressor of cutinase production, but basal levels of the enzyme could be induced at high concentrations of glucose. Induction of cutinase was inhibited by cycloheximide, but not actinomycin D. O-Methyl-O-butyl-O-(3,5,6-trichloro-2-pyridyl)phosphate was shown to act as an almost quantitative inhibitor of cutinase purified from V. inaequalis (...)