Key result
Cloned human prostacyclin receptor encodes a 386-amino acid GPCR highly expressed in cardiovascular tissues.
Why the study?
Impairment of prostacyclin receptor activity is implicated in various human cardiovascular diseases, but its molecular characteristics and gene expression in human tissues were not fully elucidated.
Population
Human lung cDNA library, COS-7 cells, and human cardiovascular tissues including aorta, lung, atrium, ventricle, and kidney
Comparison
Molecular cloning and expression analysis of human prostacyclin receptor cDNA versus no receptor characterization
Design
Molecular cloning and gene expression study using transient expression in COS-7 cells and Northern blotting
Authors
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Cloning enables molecular dissection of prostacyclin signaling; leaves open any clinical role in cardiovascular disease.
The successful cloning of the human prostacyclin receptor cDNA provides a molecular foundation for understanding its role in cardiovascular physiology and disease.
Nakagawa et al. (1994) studied this question. Molecular cloning of human prostacyclin receptor cDNA was evaluated on Isolation and characterization of human prostacyclin receptor cDNA and its gene expression. The human prostacyclin receptor cDNA was successfully cloned, encoding a 386-amino acid G protein-coupled receptor that is abundantly expressed in the aorta, lung, atrium, ventricle, and kidney.
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