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Using the Taenzer-Unna acid orcein method of staining, it was demonstrated on formalin fixed tissues that orcein reactivity is not prevented by measures which block carboxyl, hydroxyl, amine or aldehyde groups when it combines with collacin and elastic tissue, and that collagen, ordinarily unreactive, reacts strongly when these polar groups are blocked.
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Fullmer et al. (1956) studied this question.
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