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1) Ookinetes of H. gallinacea and P. cynomolgi bastianellii were studied in thin sections by electron microscopy. 2) A two-layered envelope, differentiated anteriorly into a slit with thick lips, surrounds the parasite; but there is no micropyle. Just internal to the envelope lie the hollow peripheral fibrils, 55 to 65 in number. 3) The cytoplasm contains mitochondria, lysosomes and prominent crystalloids. 4) The nucleus has no apparent membrane. 5) Penetration of the mucosal cell is described, and the intracellular location of the ookinete at this stage of its journey has been established.
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Garnhám et al. (1962) studied this question.
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