Importance of the unsaturated fatty acyl group of phospholipids in their stimulatory role on rat adrenal mitochondrial steroidogenesis

We have investigated the relationship between chemical properties of various phospholipids and their steroidogenic activity for adrenal mitochondria prepared from dexamethasone/cycloheximide-treated quiescent rats. Phospholipids studied include those purified from bovine and rat adrenal mitochondria, obtained from commercial sources, and reduced by catalytic hydrogenation. All phospholipids were subjected to analysis of their fatty acyl groups and examined for their steroidogenic activities. From these experiments, we came to the following conclusions: The degree of unsaturation in the fatty acyl moiety correlates with their steroidogenic activities regardless of head groups. Namely, polyunsaturation appears to be more important than monounsaturation with a relative insensitivity toward their head groups. Saturated phospholipids exhibit an inhibition for steroidogenic activity. Cardiolipins, which are steroidogenic, appear exceptional. Their head groups may partially participate in the activity in addition to their high content of unsaturated fatty acids. The importance of the adrenoyl (C22:4) group in phospholipids is suggested.