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The membranes of yeast mitochondria were separated and analyzed for lipid content. The sterolto‐phospholipid molar ratio was found to be very similar between the inner and outer membranes (1∶30). These observed ratios could be substantially altered by using a crude mitochondrial pellet contaminated with a “floating lipid layer”. In this case, the sterol‐to‐phospholipid molar ratios were 1∶8 to 1∶26 for the outer and inner mitochondrial membranes, respectively.
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Bottema et al. (1980) studied this question.
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