Key result
The Virogen Rotatest latex agglutination test demonstrated 87.8% sensitivity and 73.3% specificity for detecting bovine rotavirus antigen compared to the virus isolation gold standard.
Why the study?
Does the Virogen Rotatest latex agglutination test accurately detect bovine rotavirus antigen in fecal samples from diarrheic calves compared to virus isolation and ELISA?
Observational (n=63)
Does the Virogen Rotatest latex agglutination test accurately detect bovine rotavirus antigen in fecal samples from diarrheic calves compared to virus isolation and ELISA?
The Virogen Rotatest latex agglutination test is a highly efficient, rapid screening tool for detecting bovine rotavirus in clinical laboratories.
The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect the presence of group A rotavirus antigen. Of the 63 samples screened by VI-FA, 33 (58%) tested positive for rotavirus antigen. When the results from the LAT were compared to those from VI-FA, the "gold standard" for detection of bovine rotavirus in fecal samples, the sensitivity and specificity were found to be 87.8 and 73.3%, respectively. Latex agglutination compared with ELISA (the reference method) showed 100% sensitivity and 96.3% specificity, and when ELISA was compared with VI, the sensitivity was 84.8% and the specificity was 73.3%. Latex agglutination is easy to perform in a short time and does not require expensive equipment or skilled personnel, and the reagents have long shelf lives. These factors make the LAT suitable and highly efficient for use in a clinical laboratory as a rapid screening test for bovine rotavirus.
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Al-Yousif et al. (2001) conducted an observational in Bovine rotavirus (n=63). Virogen Rotatest latex agglutination test (LAT) vs. Virus isolation (VI-FA) and ELISA was evaluated on Detection of bovine rotavirus antigen. The Virogen Rotatest latex agglutination test demonstrated 87.8% sensitivity and 73.3% specificity for detecting bovine rotavirus antigen compared to the virus isolation gold standard.
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