Enzyme-filled stable polymeric capsules (polymersomes) that are permeable to low-molecular-weight (pro-fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build-up of fluorescent product that is prevented from leaking out by co-encapsulation of a trapping agent.
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Nallani et al. (2009) studied this question.
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