Reactive groups on polymer covered electrodes, 4. Lactate‐oxidase‐biosensor based on electrodes modified by polythiophene

Electrocopolymerization of 3‐thiopheneacetic acid (1) and 3‐methylthiophene (2) under various conditions produces poly{(3‐methylthiophene‐2,5‐diyl)‐co‐[3‐(carboxymethyl)thiophene‐2,5‐diyl]} (3). By activation of the carboxy groups with dicyclohexylcarbodiimide (DCC) lactate oxidase (LOD) is covalently bonded to the surface of the electrode. In this way a lactate sensor was formed which is applicable for the determination of lactate in micromolar concentrations.