The mode of development of La Crosse virus in blood meal-infected Aedes triseriatus was determined by fluorescent-antibody technique. Midguts contained large amounts of antigcn by 7 days extrinsic incubation, and most females had disseminated infections by 14 days. Following detection in guttissues, antigen was next found in either abdominal ganglia, fat body, heart and pericardial cells, or reproductive tract tissues, suggesting spread hy hemolymph. Mosquitoes infected during their 2nd engorgement had greater amounts of disseminated antigen than those infected during initial engorgement.
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Beaty et al. (1978) studied this question.
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