Key result
Cyclic mechanical stretching of human cardiomyocytes altered gene expression profiles, with short-term stretching affecting cholesterol biosynthesis and long-term stretching affecting cell migration.
Why the study?
Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood.
Cyclical stretching alters gene expression in human cardiomyocytes, potentially impacting cardiac cell metabolism and cholesterol biosynthesis signaling in pressure-overloaded cardiomyopathy.
Does not support clinical changes in pressure-overload cardiomyopathy; leaves open duration-dependent stretch effects on cardiomyocyte gene regulation.
Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 87, 32, 29, and 28 genes were differentially expressed after 1, 4, 12, 24, and 48 hours of stretching. Furthermore, 10 of the 29 genes that were up-regulated and 11 of the 28 that were down-regulated after 24 h showed the same changes after 48 h. We then examined expression of the genes that encode serpin family E member 1 (SERPINE1), DNA-binding protein inhibitor 1 (ID1), DNA-binding protein inhibitor 3 (ID3), and CCL2, a cytokine that acts as chemotactic factor in monocytes, in an RT-PCR experiment. The same changes were observed for all four genes after all cyclic stretching durations, confirming the NGS results. Taken together, these findings suggest that cyclical stretching can alter cardiac cell physiology by activating cardiac cell metabolism and impacting cholesterol biosynthesis signaling.
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Chen et al. (2020) studied In vitro human cardiomyocytes. Cyclic stretching (15% strain, 0.5 Hz) vs. Static condition (0 hour) was evaluated on Differentially expressed genes. Cyclic mechanical stretching of human cardiomyocytes altered gene expression profiles, with short-term stretching affecting cholesterol biosynthesis and long-term stretching affecting cell migration.
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