Phospholipase A2 Hydrolysis of Mixed Phospholipid Vesicles Formed on Polyelectrolyte Hollow Capsules

Mixtures of the phospholipids L-alpha-dimyristoylphosphatidic acid (DMPA) and L-alpha-dipalmitoylphosphatidylcholine (DPPC) have been successfully adsorbed onto the charged surface of multilayer polyelectrolyte capsules to form a novel vesicle. Leaving such vesicles in phospholipase A(2) solution, we observed the hydrolysis reaction on the surface of the lipid/polymer vesicles and a permeability change before and after the reaction by confocal-laser scanning microscopy (CLSM). A capsule with adjustable permeability was constructed. This method may provide new features for drug-release vesicles.