The novel application of azulene as a visual monitor of column chromatography performance during fractionation of complex waste water extracts for measurement of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) at part‐per‐trillion concentrations is described. TCDD elutes directly behind azulene, the blue visual aid, in the 6% ethyl ether/hexane fraction during Florisil column chromatography.
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Nowicki et al. (1981) studied this question.
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