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The use of applicator strips on isoelectric focusing in immobilized pH gradient gels often results in lateral sample spreading and mixing of adjacent samples. Both effects can be reduced or eliminated if a modified applicator strip with slits at the bottom is used. Different modes of sample application were compared using the group specific component system with different concentrations of bovine serum albumin in the samples.
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Pflug et al. (1987) studied this question.
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