Serine‐Protease‐Assisted Synthesis of Peptide Substrates for α‐Chymotrypsin

Abstract δ‐Chymotraypsin catalyzes peptide‐bond formation between acylated amino‐acid and peptide esters as the carboxyl component and amino‐acid and peptide amides as the amino component. The conditions under which enzyme‐catalyzed coupling can be used for fragment condensation in peptide synthesis is investigated. To illustrate the method the synthesis of tetra‐, penta‐ and hexapeptides of the structure Ac‐Lxn …︁ LxlLyl …︁ LymNH2 with Lxl  Tyr, designed as substrates for α‐chymotrypsin, is described.