The Spatiotemporal Regulation of Glucose Metabolism in Hematopoietic Stem Cells

This is the authors' abstract. We don't add key points for this paper.

Hematopoietic stem cell fate decisions are tightly coupled to dynamic changes in glucose metabolism, which are precisely orchestrated across both the spatial and temporal dimensions during differentiation. Tracking the metabolic dynamics is crucial for understanding fate decisions of hematopoietic stem cells and facilitating their efficient expansion for therapeutic applications. Genetically encoded fluorescent biosensors have emerged as powerful tools to monitor glucose metabolism in living hematopoietic stem and precursor cells with high spatiotemporal resolution, enabling the in situ, real-time analysis of cellular metabolic heterogeneity at single-cell resolution and the analysis of the regulatory mechanisms underlying hematopoietic functions. The insights revealed by biosensors are often difficult to attain with conventional bulk or end point assays. This review underscores the unique advantages and growing promise of genetically encoded sensors in decoding the metabolic regulation of HSC differentiation, while critically examining current challenges toward their broader application in stem cell biology.