Key result
Single-cell RNA sequencing of neonatal mouse hearts generated an atlas of 10,000 cardiomyocytes, revealing unexpected erythrocyte-like terminal cardiomyocytes and cardiomyocyte-like conducting cells.
Why the study?
Application of single-cell RNA sequencing in cardiomyocytes has been dissatisfactory because cardiomyocytes are too large and fragile to withstand traditional single-cell approaches.
Provides a comprehensive single-cell atlas of neonatal mouse hearts, revealing novel cardiomyocyte subpopulations including erythrocyte-like terminal cardiomyocytes.
BACKGROUND: Single-cell RNA sequencing is widely used in cancer research and organ development because of its powerful ability to analyze cellular heterogeneity. However, its application in cardiomyocytes is dissatisfactory mainly because the cardiomyocytes are too large and fragile to withstand traditional single-cell approaches. METHODS AND RESULTS: Through designing the isolation procedure of neonatal mouse cardiac cells, we provide detailed cellular atlases of the heart at single-cell resolution across 4 different stages after birth. We have obtained 10 000 cardiomyocytes; to our knowledge, this is the most extensive reference framework to date. Moreover, we have discovered unexpected erythrocyte-like cardiomyocyte-terminal cardiomyocytes, comprising more than a third of all cardiomyocytes. Only a few genes are highly expressed in these cardiomyocytes. They are highly differentiated cardiomyocytes that function as contraction pumps. In addition, we have identified 2 cardiomyocyte-like conducting cells, lending support to the theory that the sinoatrial node pacemaker cells are specialized cardiomyocytes. Notably, we provide an initial blueprint for comprehensive interactions between cardiomyocytes and other cardiac cells. CONCLUSIONS: This mouse cardiac cell atlas improves our understanding of cardiomyocyte heterogeneity and provides a valuable reference in response to varying physiological conditions and diseases.
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Shen et al. (2023) studied Neonatal mouse cardiac cells (n=10,000). Single-cell RNA sequencing was evaluated on Cellular atlas of the heart at single-cell resolution. Single-cell RNA sequencing of neonatal mouse hearts generated an atlas of 10,000 cardiomyocytes, revealing unexpected erythrocyte-like terminal cardiomyocytes and cardiomyocyte-like conducting cells.
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