Key result
Inclusion of large concentrations of permeable weak electrolytes in the pipette solution enables predictable and reversible manipulation of intracellular pH in whole cell or perforated-patch modes.
A simple procedure using permeable weak electrolytes in the pipette solution enables predictable and reversible manipulation of intracellular pH in cells during whole cell or perforated-patch clamping.
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Enables pH control in patch-clamp experiments; leaves open validation and utility in cardiovascular cell models.
Grinstein et al. (1994) studied this question. Inclusion of large concentrations of permeable weak electrolytes in the pipette solution was evaluated on Manipulation of intracellular pH (pHi). Inclusion of large concentrations of permeable weak electrolytes in the pipette solution enables predictable and reversible manipulation of intracellular pH in whole cell or perforated-patch modes.
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