In Vivo Chemoenzymatic Control of N‐Terminal Processing in Recombinant Human Epidermal Growth Factor

Excision of N-terminal residues in human epidermal growth factor during recombinant protein expression can be influenced by substitution of methionine (Met) with homopropargylglycine (Hpg) or azidohomoalanine (Aha). This extends the natural rules of N-terminal residue excision and opens up the possibility for chemoenzymatic control of this co-translational modification.