Redox Signaling Induced Laminin Receptor RPSA Mediates Cell Adhesion Following Radiofrequency Glow Discharge Treatments

This is the authors' abstract. We don't add key points for this paper.

Current biomaterials are effective at replacing biological structures, but are limited by infections and long-term material failures. In this study, we examined the molecular mechanisms of Radio Frequency Glow Discharge Treatments (RFGDT) in mediating disinfection of biomaterial surfaces and concurrently promoting cell attachment and proliferation. Dental biomaterials were subjected to RFGDT and viability of oral microbial species namely Streptococcus mutants (SM), Streptococcus gordonii (SG), Moraxella catarrhalis (MC), and Porphyromonas gingivalis (PG) were assessed. Cell attachment and survival of a pre-odontoblast cell line, MDPC-23, was examined. Finally, mechanistic investigations into redox generation and biological signaling were investigated. Dental biomaterials induced reactive ozygen species (ROS) following dose-dependent RFGDT based on their compositions. RFGDT reduced microbial viability in the catalase-negative (SM and SG) species more effectively than catalase-positive (MC and PG) species. Cell adhesion assays noted improved MDPC-23 attachment and survival. Pretreatments with N-acetylcysteine (NAC) and catalase abrogated these responses. Immunoassays noted redox-induced downstream expression of a laminin receptor, RPSA, following RFGDT. Thus, RFGDT-induced redox mediates antimicrobial and improved cell responses such as adhesion and proliferation. These observations together provide a mechanistic rationale for the clinical utility of RFGDT with dental biomaterials for regenerative clinical applications.