In vitro study demonstrates reduced UVB photodamage in human keratinocytes via Staphylococcus epidermidis supernatant, highlighting postbiotics for skin photoprotection.
Key Points
To evaluate the photoprotective activity and molecular mechanisms of cell-free supernatant from the skin commensal Staphylococcus epidermidis CCSM0287 against UVB-induced photodamage and barrier impairment.
Treated UVB-irradiated HaCaT human keratinocytes with cell-free supernatant derived from S. epidermidis CCSM0287 (SE 287-CFS).
Identified functional metabolites using non-targeted metabolomics coupled with correlation analysis.
SE 287-CFS significantly decreased ROS, MDA, and pro-inflammatory cytokines while increasing antioxidant enzyme activity, IL-10 levels, and transepithelial electrical resistance in irradiated HaCaT cells.
Treatment suppressed the ROS/MAPK/NF-κB signaling axis by blocking p65 nuclear translocation and stimulated keratinocyte proliferation and migration.
Metabolomic profiling identified butyric and adipic acids as drivers of antioxidant and anti-inflammatory effects, alongside succinic, acetic, and propanoic acids linked to barrier repair.