Single-molecule insights into SAM-I riboswitch ligand binding using scaled magnetic force spectroscopy with comparison to conventional biophysical methods

Q: What does this research mean for the field?

SAM and SAH binding impact the conformation dynamics of the SAM-I riboswitch differently, indicating distinct stabilization mechanisms between the two ligands. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Zhen Wang; Henning Labuhn; Rémi Moulinas; David Salthouse; Akifumi Nakamura; Yoshihiro Akahori; Airi Kon; Shohei Kawasaki; Adeline Feri; Pascale Beurdeley

doi:10.1080/15476286.2026.2705059

September 2, 2026RNA BiologyOpen Access

Single-molecule insights into SAM-I riboswitch ligand binding using scaled magnetic force spectroscopy with comparison to conventional biophysical methods

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Key Points

Determine how SAM and SAH binding differentially affect the conformational dynamics and mechanical stability of the SAM-I riboswitch.
Applied scaled magnetic force spectroscopy to subject SAM-I riboswitch RNA to constant forces across extended periods.
Tracked and compared the conformational dynamics of RNA complexes formed with SAM versus SAH ligands.
Application of constant mechanical force demonstrates distinct conformational dynamics in SAM-I riboswitches upon SAM binding compared to SAH binding.
Ligand-dependent mechanical responses indicate fundamentally different stabilization mechanisms between SAM and SAH interactions.

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Implication

Single-molecule force spectroscopy reveals distinct conformational dynamics induced by SAM versus SAH in SAM-I riboswitches, highlighting divergent RNA stabilization mechanisms.

Abstract

-adenosylhomocysteine (SAH) binding. By subjecting the RNA to constant forces for extended periods, we were able to observe that the conformation dynamics of SAM-I are impacted differently by SAM and SAH binding, pointing to different stabilization mechanisms between the two ligands.

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Wang et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd49d6b95aff0620ec5d1 https://doi.org/10.1080/15476286.2026.2705059

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Single-molecule insights into SAM-I riboswitch ligand binding using scaled magnetic force spectroscopy with comparison to conventional biophysical methods

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Single-molecule insights into SAM-I riboswitch ligand binding using scaled magnetic force spectroscopy with comparison to conventional biophysical methods

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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