What question did this study set out to answer?

To identify the specific 68-kd target antigen bound by serum antibodies in patients presenting with idiopathic, progressive, bilateral sensorineural hearing loss.

October 1, 1995Archives of Otolaryngology - Head and Neck Surgery

Serum Antibodies to Heat Shock Protein 70 in Sensorineural Hearing Loss

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Authors

DBDonald B. BlochMassachusetts General Hospital JMJ. E. San Martin SRS. Rauch

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Implication

Biochemical analysis reveals serum antibodies target heat shock protein 70 in progressive bilateral sensorineural hearing loss, indicating an autoimmune component to inner ear damage.

Key Points

To identify the specific 68-kd target antigen bound by serum antibodies in patients presenting with idiopathic, progressive, bilateral sensorineural hearing loss.
Purified the 68-kd target protein from renal extracts using gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis.
Digested the isolated protein with trypsin and fractionated the resulting peptide fragments using high-performance liquid chromatography for amino acid sequencing.
Assessed serum antibody reactivity from six patients alongside monoclonal antibodies to constitutive and stress-inducible HSP70 against renal extracts using Western blotting.
A high-performance liquid chromatography fraction containing a 2776-Da peptide revealed a 22-amino-acid sequence identical to residues 424 through 445 of heat shock protein 70 (HSP70).
Monoclonal antibody directed against HSP70 demonstrated specific binding reactivity with the purified 68-kd antigen on Western blot analysis.
Sera from all six evaluated patients exhibited preferential antibody reactivity against HSP70 in renal tissue extracts.

Cite This Study

Bloch et al. (1995) studied this question.

synapsesocial.com/papers/6a9be0128fb05164be8508bb https://doi.org/10.1001/archotol.1995.01890100075013

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Implication

Biochemical analysis reveals serum antibodies target heat shock protein 70 in progressive bilateral sensorineural hearing loss, indicating an autoimmune component to inner ear damage.

Serum Antibodies to Heat Shock Protein 70 in Sensorineural Hearing Loss

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Implication

Key Points

Cite This Study

Serum Antibodies to Heat Shock Protein 70 in Sensorineural Hearing Loss

Discussion

Member takes

Implication

Key Points

Cite This Study

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