Fluorescent microscopy of sunflower glandular trichomes from anther appendages showed a strong autofluorescence, caused by flavonoids. Chemical analysis with NMR and HRMS lead to the identification of 7-hydroxy-6,8,4′-trimethoxyflavone, a novel 5-deoxyflavone, exclusively found in capitate glandular trichomes. 5-Deoxy flavones are rarely found in Asteraceae and this is the first report for the genus Helianthus. Semiquantitative RT-PCR showed the presence of transcripts for phenylalanine ammonia lyase (PAL) and chalcone synthase (CHS) in all biosynthetically active trichome stages. PAL and CHS are key steps in flavonoid biosynthesis indicating that flavonoid production occurs directly within the trichomes. This offers the possibility for examination of 5-deoxyflavone biosynthesis within Asteraceae.
No takes yet. Share an insight, caveat, or question.
Göpfert et al. (2006) studied this question.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: