Tropolone was isolated from the shake cultured medium of a plant pathogenic Pseudomonas sp., and was found to act as a root growth-inhibitor. It can be used for the detection and the identification of the pathogen. Recently, seedling blight of rice grown in nursery boxes has occurred annually in the northeastern part of Chiba prefecture, Japan. Root growth of diseased seedlings is seriously impaired and the seedlings easily tumble over, hence the symptoms are referred to as •gkorobi-nae (seedling tumbling)•h. When the growth of the affected seedlings exceeds several centimeters, chlorosis occurs at the basal parts of the leaves. Then, the seedlings wither and become brown, but never rot. The Omigawa isolate of Pseudomonas sp. named after the town from which it was first isolated, was shown to be the causal agent of the disease, and was related to P.
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Azegami et al. (1985) studied this question.