Glycoprotein 130 Antagonism Counteracts Metabolic and Inflammatory Alterations to Enhance Right Ventricle Function in Pulmonary Artery Banded Pigs

Figures

• 

  Figure 1:. GP130 antagonism enhanced RV function and mitigated cardiomyocyte hypertrophy. (A) Representative still images from terminal cMRI studies. (B) Treatment with SC144 significantly enhanced RV function compared to PAB-Veh pigs (control: 60±4%, PAB-Veh: 22±10%, PAB-SC144: 37±6%) (C) Afterload was similar between PAB-Veh and PAB-SC144 pigs (PAB-Veh: 31±10, PAB-SC144: 31±5). (D) Pulmonary artery constriction was comparable in PAB-Veh and PAB-SC144 pigs (PAB-Veh: 89±2%, PAB-SC144: 86±5%). (E) Representative confocal microscopy images of RV sections stained with wheat germ agglutinin (green: WGA, blue: DAPI) to evaluate cardiomyocyte hypertrophy. (F) Treatment with SC144 prevented cardiomyocyte hypertrophy (control: 131±54 μm2, PAB-Veh: 200±70 μm2, PAB-SC144: 129±62 μm2). Kruskal-Wallis ANOVA with Dunn post hoc test in F.

• 

  Figure 2:. snRNAseq identified alterations in the cellular landscape in the three experimental groups. (A) Steps of single-nucleus RNA sequencing (snRNAseq) analysis. (B) Uniform manifold approximation and projection (UMAP) visualization of 8 cell types identified with unsupervised clustering. (C) Canonical markers used to validate cell type identities. UMAP and relative abundances of each cell type in (D) control, (E) PAB-Veh, and (F) PAB-SC144 RVs. (G) Correlation analysis of relative abundance and ejection fraction (EF). The relative abundances of macrophages, lymphocytes, and pericytes were significantly negatively associated with EF, and cardiomyocyte abundance was significantly positively correlated to EF. (H) Principal component analysis separated experimental groups using nuclei relative abundance. Differential expression analysis of pseudobulked data from all 8 cell types (red: Log2FoldChange>0.5 and adjusted p-value<0.05, blue: Log2FoldChange<−0.5 and adjusted p-value<0.05) comparing (I) PAB-Veh vs control, (J) PAB-SC144 vs control, and (K) PAB-SC144 vs PAB-Veh. The comparison between PAB-Veh and control had the most DEGs, and cardiomyocytes had the most DEGs across comparisons.

• 

  Figure 3:. Cardiomyocyte metabolic genetic programming was altered by PAB but partially rescued with SC144 treatment. (A) UMAP displaying 8 subgroups of cardiomyocytes (CM). (B) Percent of CM nuclei from each pig across subgroups. (C) UMAP visualizing changes in cardiomyocyte subclusters between experimental groups. (D) Correlation analysis of relative abundance of cardiomyocyte subclusters and ejection fraction (EF). CM3 was negatively correlated to EF and CM2 was positively correlated to EF. Pathway analysis of DEGs (E) increased and (F) decreased in PAB-Veh nuclei compared to control. Pathways of DEGs (G) elevated and (H) decreased in PAB-SC144 compared to control. Metabolic pathways were altered by PAB and treatment with SC144, and downstream GP130 signaling was reduced in PAB-SC144 compared to control. (I) Pathway analysis of DEGs upregulated in PAB-SC144 compared to PAB-Veh. There were no enriched pathways comparing DEGs downregulated in PAB-SC144 and PAB-Veh.

• 

  Figure 4:. SC144 counteracted mTORC1-associated autophagic alterations. (A) Schematic of phosphoproteomics analysis. (B) Two (MAPK, PRKCZ, italicized, red stars) of the top 10 kinases identified in PAB-Veh were downstream of GP130. The mTORC1 subunit, RPS6KB1 (red italics) was also predicted to be enriched. (C) In PAB-SC144 RVs, several polo-like kinases (PLK, red italics), which antagonize mTORC1 were predicted to be enriched. (D) Primary steps in autophagy. Contents are isolated in an autophagosome, which fuses with a lysosome where the products are degraded and recycled. mTORC1 inhibits the initiation of autophagy. The autophagy inhibitor, bafilomycin (BAF), prevents lysosome and autophagosome fusion. Light chain 3 (LC3) is a component of autophagosomes. Porcine RV proteomics of the (E) autophagy, (F) mitophagy, and (G) lysosome pathways revealed many proteins were downregulated in PAB-Veh, but normalized with SC144 treatment. (H) Human PAH patients with decompensated RV function (dRV) had mostly decreased abundances of autophagy pathway proteins. (I) Mitophagy pathway proteins were mostly upregulated in dRV patients. (J) dRV patients had primarily decreased abundances of lysosome…

• 

  Figure 5:. GP130 inhibition normalized mitochondrial dimensions, cristae morphology and mitigated dysregulation of oxidative phosphorylation and fatty acid metabolism proteins. (A) Representative electron micrograph demonstrating PAB-Veh mitochondria are smaller and more fragmented (white arrows). GP130 antagonism with SC144 normalized (B) mitochondrial shape assessed by length to width ratio (control: 1.8±0.7, PAB-Veh: 1.6±0.6, PAB-SC144: 1.9±0.9), and (C) mitigated changes in cristae abundance (control: 1.7±1, PAB-Veh: 1.4±1, PAB-SC144: 1.5±1). (D) Hierarchical cluster analysis and (E) partial least squares discriminant analysis of RV mitochondrial enrichment proteomics cluster PAB-SC144 clusters with control (F) Pathway analysis identified 6 of the top 20 pathways are related to metabolism (italicized, red star). (G) Proteins in complexes I-V of the electron transport chain were downregulated in PAB-Veh mitochondria, and normalized with SC144 treatment. (H) Schematic of complexes I-V of the electron transport chain. Red arrows (PAB-Veh) and blue equal signs (PAB-SC144) visualize changes compared to control abundances. (I) Proteins in the mitochondrial beta-oxidation pathway were reduced…

• 

  Figure 6:. Combined lipidomics and metabolomics analyses identified altered RV metabolism. (A) PAB-Veh and PAB-SC144 had a reduction in the total percentage of triacylglycerols (TAG, control: 21%, PAB-Veh: 1%, PAB-SC144: 3%), and an increase in phosphatidylcholines (PC, control: 32%, PAB-Veh: 41%, PAB-SC144: 40%) and phosphatidylethanolamines (PE, control: 37%, PAB-Veh: 45%, PAB-SC144: 44%). (B) Hierarchical cluster analysis of lipidomics clustered PAB-Veh and PAB-SC144 groups together. (C) Random forest classification identified the top 15 lipids in distinguishing between experimental groups. 9 of 15 are TAG species. (D) Lipid Ontology enrichment analysis revealed triacylglycerols, glycerolipids, and fatty acids were the most enriched pathways. (E) Hierarchical cluster analysis of metabolomics data clustered PAB-Veh and PAB-SC144 groups together. (F) Pathway analysis of metabolomics data identified amino acid metabolism pathways account for many of the most enriched pathways. Scale bars show relative changes between samples in B,E

• 

  Figure 7:. SC144 counteracted changes in lipid and fatty acid metabolism, but did not markedly alter amino acid metabolism. (A) Central steps in the breakdown of triacylglycerols into diacylglycerols and monoacylglycerols. ATGL: adipose triacylglycerol lipase; HSL: hormone sensitive lipase; MGL: monoacylglycerol lipase (B) Abundances of triacylglycerols are reduced in PAB-Veh and PAB-SC144. (C) Diacylglycerols were similar between all groups. (D) Abundances of monoacylglycerols are elevated in PAB-Veh RVs. (E) Long chain fatty acid species are decreased in PAB-Veh RVs compared to control and PAB-SC144. (F) Abundances of medium chain fatty acids are increased in PAB-Veh pigs. (G) Most acylcarnitines are increased in PAB-Veh RVs compared to control and PAB-SC144 RVs. (H) Key proteins and metabolites of the acylcarnitine shuttle in mitochondria. Red arrows (PAB-Veh) and blue equal signs (PAB-SC144) visualize changes in these metabolites compared to control. A carnitine molecule is exchanged for the CoA of a fatty acyl-CoA, and the subsequent acylcarnitine is transported into the mitochondrial matrix. The carnitine molecule is removed, and the fatty acyl-CoA undergoes beta-oxidation. ACS:…

• 

  Figure 8:. Proposed mechanism demonstrating GP130 antagonism with SC144 improves RV function. SC144 reduces the abundance of macrophages and lymphocytes and blocks pro-inflammatory gene transcription in both cell types, rebalances autophagy and preserves fatty acid metabolism, and restores endothelial cell and pericyte communication.