Enzymatic synthesis and expressed protein ligation were combined in a method for protein synthesis in which protein thioesters with specific leaving groups could be used by protease V8 from Staphylococcus aureus to catalyze the formation of peptide bonds. This method was applied to the synthesis of fluorescence-labeled (Fl) analogues of proneuropeptide Y (proNPY; see scheme). CBD=chitin binding domain.
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Machova et al. (2003) studied this question.
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