Imaging of Endogenous Hydrogen Peroxide during the Process of Cell Mitosis and Mouse Brain Development with a Near-Infrared Ratiometric Fluorescent Probe

This is the authors' abstract. We don't add key points for this paper.

Hydrogen peroxide (H 2 O 2 ) is emerging as a new second messenger, which plays vital roles in intracellular signaling, thereby triggering physiological variations in terms of proliferation, differentiation, and migration. As known, cell mitosis has close association to the intracellular level of H 2 O 2, which contribute to the significant effects on brain development, especially during the critical period of immaturity. Unfortunately, imaging H 2 O 2 in a mammalian brain is still challenging. Herein, to further investigate the biological roles of endogenous H 2 O 2 in cell mitosis, we develop a near-infrared ratiometric fluorescent probe Cy-PFS for specifically imaging endogenous H 2 O 2 in cells and in vivo. Employing the probe Cy-PFS, we examine the critical effects of endogenous H 2 O 2 on proliferation of cells in live hippocampal neurons cells, and our results provide strong evidence for H 2 O 2 signaling in cell mitosis through growth factor signaling. Furthermore, we successfully demonstrate the close association of endogenous H 2 O 2 level changes with the brain development at various stages. We envision that this present probe has potential as a promising useful chemical imaging tool for exploring the roles of H 2 O 2 in cell mitosis.